In-Office Biological Indicators Validation

You will learn about instrument decontamination in dental practices, and to ensure safe, effective, and validated processes (including biological indicators) for instrument decontamination to ensure patient safety.

1. General principles:
- Follow guidelines and manufacturer instructions
- Use validated decontamination cycles
- Implement proper PPE and hand hygiene
- Ensure unidirectional workflow from dirty to clean areas

2. Cleaning methods:
- Instrument washer disinfectors (best practice)
- Ultrasonic cleaners 
- Manual cleaning (least preferred)

3. Sterilization:
- Class B (pre-vacuum) autoclaves are most effective
- Proper packaging and labeling of instruments
- Validation and testing of equipment is crucial

4. Key considerations:
- Proper cleaning of handpieces
- Avoiding overloading of equipment
- Maintaining records and documentation
- Regular audits and maintenance

5. COVID-19 impacts:
- Increased focus on infection control
- New pre-sterilization phase in some autoclaves to target viruses

6. Common errors:
- Operator errors more common than equipment failures
- Importance of proper training and understanding protocols

Local regulations can vary between countries and regions.